• Western blot analysis of extracts of various cell lines, using UBE4B antibody (STJ11102680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-UBE4B antibody (1-100) [S0MR] (STJ11102680)

SKU:
STJ11102680

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-UBE4B (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE4B
Gene ID: 10277
Uniprot ID: UBE4B_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4B (O95155).
Immunogen Sequence: MEELSADEIRRRRLARLAGG QTSQPTTPLTSPQRENPPGP PIAASAPGPSQSLGLNVHNM TPATSPIGASGVAHRSQSSE GVSSLSSSPSNSLETQSQSL
Tissue Specificity Expressed in differentiated myotubes (at protein level). Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
Post Translational Modifications Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Function Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.
Protein Name Ubiquitin Conjugation Factor E4 B
Homozygously Deleted In Neuroblastoma 1
Ring-Type E3 Ubiquitin Transferase E4 B
Ubiquitin Fusion Degradation Protein 2
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Ubiquitin Conjugation Factor E4 B antibody
Anti-Homozygously Deleted In Neuroblastoma 1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase E4 B antibody
Anti-Ubiquitin Fusion Degradation Protein 2 antibody
Anti-UBE4B antibody
Anti-HDNB1 antibody
Anti-KIAA0684 antibody
Anti-UFD2 antibody

Information sourced from Uniprot.org

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