![Immunofluorescence analysis of HeLa cells using [KO Validated] UBE2O Rabbit polyclonal antibody (STJ112076) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92148/372486/STJ112076_2__60125.1713138472.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] UBE2O Rabbit polyclonal antibody (STJ112076) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and UBE2O knockout (KO) HeLa cells, using [KO Validated] UBE2O Rabbit polyclonal antibody (STJ112076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92148/372487/STJ112076_3__19411.1713138473.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and UBE2O knockout (KO) HeLa cells, using [KO Validated] UBE2O Rabbit polyclonal antibody (STJ112076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit polyclonal antibody (STJ112076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92148/372488/STJ112076_4__97452.1713138474.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit polyclonal antibody (STJ112076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-UBE2O antibody (1073-1292) (STJ112076)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UBE2O (1073-1292) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:4000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE2O |
| Gene ID | 63893 |
| Uniprot ID | UBE2O_HUMAN |
| Immunogen Region | 1073-1292 |
| Immunogen Sequence | LVNEPYYNEAGFDSDRGLQE GYENSRCYNEMALIRVVQSM TQLVRRPPEVFEQEIRQHFS TGGWRLVNRIESWLETHALL EKAQALPNGVPKASSSPEPP AVAELSDSGQQEPEDGGPAP GEASQGSDSEGGAQGLASAS RDHTDQTSETAPDASVPPSV KPKKRRKSYRSFLPEKSGYP DIGFPLFPLSKGFIKSIRGV LTQFRAALLEAGMPECTEDK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). |
Additional Info
| Tissue Specificity | Predominantly expressed in skeletal muscle and heart. |
| Post Translational Modifications | Phosphorylated. Phosphorylation affects subcellular location. Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location. |
| Function | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly. |
| Protein Name | (E3-Independent E2 Ubiquitin-Conjugating EnzymeE2/E3 Hybrid Ubiquitin-Protein Ligase Ube2oUbiquitin Carrier Protein OUbiquitin-Conjugating Enzyme E2 OUbiquitin-Conjugating Enzyme E2 Of 230 KdaUbiquitin-Conjugating Enzyme E2-230kUbiquitin-Protein Ligase O |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusMainly Localizes To The Cytoplasm |
| Alternative Antibody Names | Anti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme antibodyAnti-E2/E3 Hybrid Ubiquitin-Protein Ligase Ube2o antibodyAnti-Ubiquitin Carrier Protein O antibodyAnti-Ubiquitin-Conjugating Enzyme E2 O antibodyAnti-Ubiquitin-Conjugating Enzyme E2 Of 230 Kda antibodyAnti-Ubiquitin-Conjugating Enzyme E2-230k antibodyAnti-Ubiquitin-Protein Ligase O antibodyAnti-UBE2O antibodyAnti-KIAA1734 antibody |
Information sourced from Uniprot.org