| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UBE2O (1073-1292) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:4000IF/ICC 1:50-1:200IP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBE2O |
| Gene ID: |
63893 |
| Uniprot ID: |
UBE2O_HUMAN |
| Immunogen Region: |
1073-1292 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). |
| Immunogen Sequence: |
LVNEPYYNEAGFDSDRGLQE GYENSRCYNEMALIRVVQSM TQLVRRPPEVFEQEIRQHFS TGGWRLVNRIESWLETHALL EKAQALPNGVPKASSSPEPP AVAELSDSGQQEPEDGGPAP GEASQGSDSEGGAQGLASAS RDHTDQTSETAPDASVPPSV KPKKRRKSYRSFLPEKSGYP DIGFPLFPLSKGFIKSIRGV LTQFRAALLEAGMPECTEDK |
| Tissue Specificity | Predominantly expressed in skeletal muscle and heart. |
| Post Translational Modifications | Phosphorylated. Phosphorylation affects subcellular location. Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location. |
| Function | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly. |
| Protein Name | (E3-Independent E2 Ubiquitin-Conjugating EnzymeE2/E3 Hybrid Ubiquitin-Protein Ligase Ube2oUbiquitin Carrier Protein OUbiquitin-Conjugating Enzyme E2 OUbiquitin-Conjugating Enzyme E2 Of 230 KdaUbiquitin-Conjugating Enzyme E2-230kUbiquitin-Protein Ligase O |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusMainly Localizes To The Cytoplasm |
| Alternative Antibody Names | Anti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme antibodyAnti-E2/E3 Hybrid Ubiquitin-Protein Ligase Ube2o antibodyAnti-Ubiquitin Carrier Protein O antibodyAnti-Ubiquitin-Conjugating Enzyme E2 O antibodyAnti-Ubiquitin-Conjugating Enzyme E2 Of 230 Kda antibodyAnti-Ubiquitin-Conjugating Enzyme E2-230k antibodyAnti-Ubiquitin-Protein Ligase O antibodyAnti-UBE2O antibodyAnti-KIAA1734 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
