Anti-UBE2N antibody (1-100) [S3MR] (STJ11102463)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Ube2N/Ubc13 (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S3MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE2N |
| Gene ID | 7334 |
| Uniprot ID | UBE2N_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MAGLPRRIIKETQRLLAEPV PGIKAEPDESNARYFHVVIA GPQDSPFEGGTFKLELFLPE EYPMAAPKVRFMTKIYHPNV DKLGRICLDILKDKWSPALQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ube2N/Ubc13 (P61088). |
Additional Info
| Post Translational Modifications | Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. |
| Function | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 NBendless-Like Ubiquitin-Conjugating EnzymeE2 Ubiquitin-Conjugating Enzyme NUbc13Ubch13Ubiquitin Carrier Protein NUbiquitin-Protein Ligase N |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5205685Reactome: R-HSA-5607764Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-69473Reactome: R-HSA-8866654Reactome: R-HSA-9020702Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9646399Reactome: R-HSA-9705671Reactome: R-HSA-975110Reactome: R-HSA-975144Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 N antibodyAnti-Bendless-Like Ubiquitin-Conjugating Enzyme antibodyAnti-E2 Ubiquitin-Conjugating Enzyme N antibodyAnti-Ubc13 antibodyAnti-Ubch13 antibodyAnti-Ubiquitin Carrier Protein N antibodyAnti-Ubiquitin-Protein Ligase N antibodyAnti-UBE2N antibodyAnti-BLU antibody |
Information sourced from Uniprot.org