| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S3MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2N |
| Gene ID: | 7334 |
| Uniprot ID: | UBE2N_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ube2N/Ubc13 (P61088). |
| Immunogen Sequence: | MAGLPRRIIKETQRLLAEPV PGIKAEPDESNARYFHVVIA GPQDSPFEGGTFKLELFLPE EYPMAAPKVRFMTKIYHPNV DKLGRICLDILKDKWSPALQ |
| Post Translational Modifications | Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. |
| Function | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 N Bendless-Like Ubiquitin-Conjugating Enzyme E2 Ubiquitin-Conjugating Enzyme N Ubc13 Ubch13 Ubiquitin Carrier Protein N Ubiquitin-Protein Ligase N |
| Database Links | Reactome: R-HSA-1169408 Reactome: R-HSA-168638 Reactome: R-HSA-168927 Reactome: R-HSA-202424 Reactome: R-HSA-2871837 Reactome: R-HSA-445989 Reactome: R-HSA-450302 Reactome: R-HSA-450321 Reactome: R-HSA-5205685 Reactome: R-HSA-5607764 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-5696395 Reactome: R-HSA-69473 Reactome: R-HSA-8866654 Reactome: R-HSA-9020702 Reactome: R-HSA-937039 Reactome: R-HSA-937041 Reactome: R-HSA-9646399 Reactome: R-HSA-9705671 Reactome: R-HSA-975110 Reactome: R-HSA-975144 Reactome: R-HSA-983168 |
| Cellular Localisation | Nucleus Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 N antibody Anti-Bendless-Like Ubiquitin-Conjugating Enzyme antibody Anti-E2 Ubiquitin-Conjugating Enzyme N antibody Anti-Ubc13 antibody Anti-Ubch13 antibody Anti-Ubiquitin Carrier Protein N antibody Anti-Ubiquitin-Protein Ligase N antibody Anti-UBE2N antibody Anti-BLU antibody |
Information sourced from Uniprot.org