Anti-UBE2F antibody (1-185) (STJ27140)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UBE2F (1-185) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE2F |
| Gene ID | 140739 |
| Uniprot ID | UBE2F_HUMAN |
| Immunogen Region | 1-185 |
| Immunogen Sequence | MLTLASKLKRDDGLKGSRTA ATASDSTRRVSVRDKLLVKE VAELEANLPCTCKVHFPDPN KLHCFQLTVTPDEGYYQGGK FQFETEVPDAYNMVPPKVKC LTKIWHPNITETGEICLSLL REHSIDGTGWAPTRTLKDVV WGLNSLFTDLLNFDDPLNIE AAEHHLRDKEDFRNKVDDYI KRYAR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1). |
Additional Info
| Tissue Specificity | Widely expressed (at protein level). |
| Post Translational Modifications | The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Mediates neddylation of the CUL9-RBX1 complex. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5). |
| Protein Name | Nedd8-Conjugating Enzyme Ube2fNedd8 Carrier Protein Ube2fNedd8 Protein Ligase Ube2fNedd8-Conjugating Enzyme 2Ring-Type E3 Nedd8 Transferase Ube2fUbiquitin-Conjugating Enzyme E2 F |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nedd8-Conjugating Enzyme Ube2f antibodyAnti-Nedd8 Carrier Protein Ube2f antibodyAnti-Nedd8 Protein Ligase Ube2f antibodyAnti-Nedd8-Conjugating Enzyme 2 antibodyAnti-Ring-Type E3 Nedd8 Transferase Ube2f antibodyAnti-Ubiquitin-Conjugating Enzyme E2 F antibodyAnti-UBE2F antibodyAnti-NCE2 antibody |
Information sourced from Uniprot.org