Western blot analysis of various lysates using UBE2D4 Rabbit pAb (STJ110630) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-UBE2D4 antibody (1-147) (STJ110630)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110630
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General Information

Short DescriptionRabbit polyclonal UBE2D4 (1-147) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolUBE2D4
Gene ID51619
Uniprot IDUB2D4_HUMAN
Immunogen Region1-147
Immunogen SequenceMALKRIQKELTDLQRDPPAQ CSAGPVGDDLFHWQATIMGP NDSPYQGGVFFLTIHFPTDY PFKPPKVAFTTKIYHPNINS NGSICLDILRSQWSPALTVS KVLLSICSLLCDPNPDDPLV PEIAHTYKADREKYNRLARE WTQKYAM
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D4 (NP_057067.1).

Additional Info

Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Protein Name Ubiquitin-Conjugating Enzyme E2 D4
E2 Ubiquitin-Conjugating Enzyme D4
Hbuce1
Ubiquitin Carrier Protein D4
Ubiquitin-Protein Ligase D4
Database Links Reactome: R-HSA-983168
Cellular Localisation
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 D4 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme D4 antibody
Anti-Hbuce1 antibody
Anti-Ubiquitin Carrier Protein D4 antibody
Anti-Ubiquitin-Protein Ligase D4 antibody
Anti-UBE2D4 antibody
Anti-UBCH5D antibody

Information sourced from Uniprot.org

Citations

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