• Western blot analysis of extracts of various cell lines, using UBE2D4 antibody (STJ110630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using UBE2D4 antibody (STJ110630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-UBE2D4 antibody (1-147) (STJ110630)

SKU:
STJ110630

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE2D4 (1-147) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2D4
Gene ID: 51619
Uniprot ID: UB2D4_HUMAN
Immunogen Region: 1-147
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D4 (NP_057067.1).
Immunogen Sequence: MALKRIQKELTDLQRDPPAQ CSAGPVGDDLFHWQATIMGP NDSPYQGGVFFLTIHFPTDY PFKPPKVAFTTKIYHPNINS NGSICLDILRSQWSPALTVS KVLLSICSLLCDPNPDDPLV PEIAHTYKADREKYNRLARE WTQKYAM
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Protein Name Ubiquitin-Conjugating Enzyme E2 D4
E2 Ubiquitin-Conjugating Enzyme D4
Hbuce1
Ubiquitin Carrier Protein D4
Ubiquitin-Protein Ligase D4
Database Links Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 D4 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme D4 antibody
Anti-Hbuce1 antibody
Anti-Ubiquitin Carrier Protein D4 antibody
Anti-Ubiquitin-Protein Ligase D4 antibody
Anti-UBE2D4 antibody
Anti-UBCH5D antibody

Information sourced from Uniprot.org

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