| Short Description : | Rabbit polyclonal UBA6 (1-260) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBA6 |
| Gene ID: | 55236 |
| Uniprot ID: | UBA6_HUMAN |
| Immunogen Region: | 1-260 |
| Immunogen Sequence: | MEGSEPVAAHQGEEASCSSW GTGSTNKNLPIMSTASVEID DALYSRQRYVLGDTAMQKMA KSHVFLSGMGGLGLEIAKNL VLAGIKAVTIHDTEKCQAWD LGTNFFLSEDDVVNKRNRAE AVLKHIAELNPYVHVTSSSV PFNETTDLSFLDKYQCVVLT EMKLPLQKKINDFCRSQCPP IKFISADVHGIWSRLFCDFG DEFEVLDTTGEEPKEIFISN ITQANPGIVTCLENHPHKL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4). |
| Tissue Specificity | Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis. |
| Function | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Also activates UBD/FAT10 conjugation via adenylation of its C-terminal glycine. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 6Ubiquitin-Activating Enzyme 6Monocyte Protein 4Mop-4Ubiquitin-Activating Enzyme E1-Like Protein 2E1-L2 |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Ubiquitin-Like Modifier-Activating Enzyme 6 antibodyAnti-Ubiquitin-Activating Enzyme 6 antibodyAnti-Monocyte Protein 4 antibodyAnti-Mop-4 antibodyAnti-Ubiquitin-Activating Enzyme E1-Like Protein 2 antibodyAnti-E1-L2 antibodyAnti-UBA6 antibodyAnti-MOP4 antibodyAnti-UBE1L2 antibody |
Information sourced from Uniprot.org
