Anti-UBA3 antibody (1-463) [S4101RM] (STJ11104101)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Nedd8-Activating Enzyme E1 Catalytic Subunit (1-463) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4101RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBA3 |
| Gene ID | 9039 |
| Uniprot ID | UBA3_HUMAN |
| Immunogen Region | 1-463 |
| Immunogen Sequence | MADGEEPEKKRRRIEELLAE KMAVDGGCGDTGDWEGRWNH VKKFLERSGPFTHPDFEPST ESLQFLLDTCKVLVIGAGGL GCELLKNLALSGFRQIHVID MDTIDVSNLNRQFLFRPKDI GRPKAEVAAEFLNDRVPNCN VVPHFNKIQDFNDTFYRQFH IIVCGLDSIIARRWINGMLI SLLNYEDGVLDPSSIVPLID GGTEGFKGNARVILPGMTAC IECTLELYPPQVNFPMCTI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human UBA3 (NP_003959.3). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Function | Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. |
| Protein Name | Nedd8-Activating Enzyme E1 Catalytic SubunitNedd8-Activating Enzyme E1cUbiquitin-Activating Enzyme E1cUbiquitin-Like Modifier-Activating Enzyme 3Ubiquitin-Activating Enzyme 3 |
| Database Links | Reactome: R-HSA-5607761Reactome: R-HSA-5676590Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nedd8-Activating Enzyme E1 Catalytic Subunit antibodyAnti-Nedd8-Activating Enzyme E1c antibodyAnti-Ubiquitin-Activating Enzyme E1c antibodyAnti-Ubiquitin-Like Modifier-Activating Enzyme 3 antibodyAnti-Ubiquitin-Activating Enzyme 3 antibodyAnti-UBA3 antibodyAnti-UBE1C antibody |
Information sourced from Uniprot.org