• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g U2AF1 antibody (STJ115132). Western blot was performed from the immunoprecipitate using U2AF1 antibody (STJ115132) at a dilution of 1:1000.
  • Immunohistochemistry analysis of U2AF1 in paraffin-embedded human lung cancer using U2AF1 Rabbit polyclonal antibody (STJ115132) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using U2AF1 Rabbit polyclonal antibody (STJ115132) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-U2AF1 antibody (1-100) (STJ115132)

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STJ115132

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-U2AF1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: U2AF1
Gene ID: 102724594/7307
Uniprot ID: U2AF1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1).
Immunogen Sequence: MAEYLASIFGTEKDKVNCSF YFKIGACRHGDRCSRLHNKP TFSQTIALLNIYRNPQNSSQ SADGLRCAVSDVEMQEHYDE FFEEVFTEMEEKYGEVEEMN
Function Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Protein Name Splicing Factor U2af 35 Kda Subunit
U2 Auxiliary Factor 35 Kda Subunit
U2 Small Nuclear Rna Auxiliary Factor 1
U2 Snrnp Auxiliary Factor Small Subunit
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Splicing Factor U2af 35 Kda Subunit antibody
Anti-U2 Auxiliary Factor 35 Kda Subunit antibody
Anti-U2 Small Nuclear Rna Auxiliary Factor 1 antibody
Anti-U2 Snrnp Auxiliary Factor Small Subunit antibody
Anti-U2AF1 antibody
Anti-U2AF35 antibody
Anti-U2AFBP antibody
Anti-FP793 antibody

Information sourced from Uniprot.org

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