• Immunohistochemistry analysis of TYMS in paraffin-embedded mouse testis using TYMS Rabbit polyclonal antibody (STJ112471) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TYMS in paraffin-embedded human thyroid cancer using TYMS Rabbit polyclonal antibody (STJ112471) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TYMS Rabbit polyclonal antibody (STJ112471) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.

Anti-TYMS antibody (1-313) (STJ112471)

SKU:
STJ112471

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TYMS (1-313) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TYMS
Gene ID: 7298
Uniprot ID: TYSY_HUMAN
Immunogen Region: 1-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human TYMS (NP_001062.1).
Immunogen Sequence: MPVAGSELPRRPLPPAAQER DAEPRPPHGELQYLGQIQHI LRCGVRKDDRTGTGTLSVFG MQARYSLRDEFPLLTTKRVF WKGVLEELLWFIKGSTNAKE LSSKGVKIWDANGSRDFLDS LGFSTREEGDLGPVYGFQWR HFGAEYRDMESDYSGQGVDQ LQRVIDTIKTNPDDRRIIMC AWNPRDLPLMALPPCHALCQ FYVVNSELSCQLYQRSGDMG LGVPFNIASYALLTYMIAH
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Protein Name Thymidylate Synthase
Ts
Tsase
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-69205
Cellular Localisation Nucleus
Cytoplasm
Mitochondrion
Mitochondrion Matrix
Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Thymidylate Synthase antibody
Anti-Ts antibody
Anti-Tsase antibody
Anti-TYMS antibody
Anti-TS antibody
Anti-OK antibody
Anti-SW-cl.29 antibody

Information sourced from Uniprot.org

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