| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Twinfilin-2 (1-349) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TWF2 |
| Gene ID: |
11344 |
| Uniprot ID: |
TWF2_HUMAN |
| Immunogen Region: |
1-349 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human TWF2 (NP_009215.1). |
| Immunogen Sequence: |
MAHQTGIHATEELKEFFAKA RAGSVRLIKVVIEDEQLVLG ASQEPVGRWDQDYDRAVLPL LDAQQPCYLLYRLDSQNAQG FEWLFLAWSPDNSPVRLKML YAATRATVKKEFGGGHIKDE LFGTVKDDLSFAGYQKHLSS CAAPAPLTSAERELQQIRIN EVKTEISVESKHQTLQGLAF PLQPEAQRALQQLKQKMVNY IQMKLDLERETIELVHTEPT DVAQLPSRVPRDAARYHFF |
| Tissue Specificity | Ubiquitously expressed (at protein level). |
| Post Translational Modifications | In vitro, phosphorylated by PRKCZ, CK2 and SRC. |
| Function | Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. |
| Protein Name | Twinfilin-2A6-Related ProteinHa6rpProtein Tyrosine Kinase 9-LikeTwinfilin-1-Like Protein |
| Database Links | Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | CytoplasmCytoskeletonPerinuclear RegionCell ProjectionStereociliumPerinuclear And G-Actin-Rich Cortical Actin Structure Sublocalization |
| Alternative Antibody Names | Anti-Twinfilin-2 antibodyAnti-A6-Related Protein antibodyAnti-Ha6rp antibodyAnti-Protein Tyrosine Kinase 9-Like antibodyAnti-Twinfilin-1-Like Protein antibodyAnti-TWF2 antibodyAnti-PTK9L antibodyAnti-MSTP011 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89709/358191/STJ11100773_1__48709.1713122506.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89710/358192/STJ11100774_1__12392.1713122508.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
