• Western blot analysis of extracts of various cell lines, using FTO antibody (STJ11101469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-FTO antibody (1-349) (STJ11101469)

SKU:
STJ11101469

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FTO (1-349) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-349
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of zebrafish FTO (XP_009301626.1).
Immunogen Sequence: MKPRQRKQYFRNMKRSDDSE REKRRKRRRLLQELGEQRIP YLSPTDPGFQDLWDSSYAGL ALRQSGTLPEGLHEKVQSAL LTLQRHGCLLRDLVRVRDRD VFTAVSRALVGQPGYTYRYL DTRLFTIPWHCEGEEGQKDE KGKPCCDSDLRDACKALWEL NQFFCSDVKQQTNARGVKRT RSDTENSEDAPGEGMCEEES VKDRLVEEKTIEEEEEDSGQ GCSHSSPPSSTPPAAQASL
Background This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A) , which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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