![Western blot analysis of lysates from wild type (WT) and TTLL12 knockout (KO) 293T cells, using [KO Validated] TTLL12 Rabbit polyclonal antibody (STJ11100870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89804/358271/STJ11100870_1__29815.1713122614.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TTLL12 knockout (KO) 293T cells, using [KO Validated] TTLL12 Rabbit polyclonal antibody (STJ11100870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and TTLL12 knockout (KO) 293T cells, using [KO Validated] TTLL12 Rabbit polyclonal antibody (STJ11100870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89804/358271/STJ11100870_1__29815.1713122614.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TTLL12 knockout (KO) 293T cells, using [KO Validated] TTLL12 Rabbit polyclonal antibody (STJ11100870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-TTLL12 antibody (485-644) (STJ11100870)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TTLL12 (485-644) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TTLL12 |
| Gene ID | 23170 |
| Uniprot ID | TTL12_HUMAN |
| Immunogen Region | 485-644 |
| Immunogen Sequence | LFVYDVFWLRFSNRAFALND LDDYEKHFTVMNYDPDVVLK QVHCEEFIPEFEKQYPEFPW TDVQAEIFRAFTELFQVACA KPPPLGLCDYPSSRAMYAVD LMLKWDNGPDGRRVMQPQIL EVNFNPDCERACRYHPTFFN DVFSTLFLDQPGGCHVTCLV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 485-644 of human TTLL12 (NP_055955.1). |
Additional Info
| Tissue Specificity | Expressed in the basal layer of prostate and endothelial cells. Increased expression in prostatic intraepithelial neoplasia and metastatic lesions. |
| Function | Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues. Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability. During RNA virus-mediated infection, acts as a negative regulator of the RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE. |
| Protein Name | Tubulin--Tyrosine Ligase-Like Protein 12Inactive Tubulin--Tyrosine Ligase-Like Protein 12 |
| Database Links | Reactome: R-HSA-8955332 |
| Cellular Localisation | CytoplasmMidbodyCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleNucleusPredominantly Localizes In The CytoplasmPartially Colocalizes With Vimentin In Prostate Cancer Cells |
| Alternative Antibody Names | Anti-Tubulin--Tyrosine Ligase-Like Protein 12 antibodyAnti-Inactive Tubulin--Tyrosine Ligase-Like Protein 12 antibodyAnti-TTLL12 antibodyAnti-KIAA0153 antibody |
Information sourced from Uniprot.org