• Immunofluorescence analysis of A549 cells using TTF2 antibody (STJ110062). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TTF2 antibody (STJ110062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-TTF2 antibody (1-270) (STJ110062)

SKU:
STJ110062

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TTF2 (1-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TTF2
Gene ID: 8458
Uniprot ID: TTF2_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTF2 (NP_003585.3).
Immunogen Sequence: MEEVRCPEHGTFCFLKTGVR DGPNKGKSFYVCRADTCSFV RATDIPVSHCLLHEDFVVEL QGLLLPQDKKEYRLFFRCIR SKAEGKRWCGSIPWQDPDSK EHSVSNKSQHASETFHHSSN WLRNPFKVLDKNQEPALWKQ LIKGEGEEKKADKKQREKGD QLFDQKKEQKPEMMEKDLSS GLVPKKKQSVVQEKKQEEGA EIQCEAETGGTHKRDFSEIK SQQCQGNELTRPSASSQEK
Function DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
Protein Name Transcription Termination Factor 2
Lodestar Homolog
Rna Polymerase Ii Termination Factor
Transcription Release Factor 2
F2
Huf2
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic During Interphase
Relocates To The Nucleus As Cells Enter Mitosis
Alternative Antibody Names Anti-Transcription Termination Factor 2 antibody
Anti-Lodestar Homolog antibody
Anti-Rna Polymerase Ii Termination Factor antibody
Anti-Transcription Release Factor 2 antibody
Anti-F2 antibody
Anti-Huf2 antibody
Anti-TTF2 antibody

Information sourced from Uniprot.org

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