• Immunohistochemistry analysis of TSN in paraffin-embedded Human colon carcinoma using TSN Rabbit polyclonal antibody (STJ11100537) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TSN in paraffin-embedded Rat ovary using TSN Rabbit polyclonal antibody (STJ11100537) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TSN in paraffin-embedded Mouse spinal cord using TSN Rabbit polyclonal antibody (STJ11100537) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TSN Rabbit polyclonal antibody (STJ11100537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TSN antibody (50-150) (STJ11100537)

SKU:
STJ11100537

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSN (50-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSN
Gene ID: 7247
Uniprot ID: TSN_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TSN (NP_004613.1).
Immunogen Sequence: GFQDIPKRCLKAREHFGTVK THLTSLKTKFPAEQYYRFHE HWRFVLQRLVFLAAFVVYLE TETLVTREAVTEILGIEPDR EKGFHLDVEDYLSGVLILAS E
Function DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots. Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.
Protein Name Translin
Component 3 Of Promoter Of Risc
C3po
Database Links Reactome: R-HSA-426486
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Translin antibody
Anti-Component 3 Of Promoter Of Risc antibody
Anti-C3po antibody
Anti-TSN antibody

Information sourced from Uniprot.org

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