Anti-TRUB1 antibody (100-280) (STJ11103344)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRUB1 (100-280) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRUB1 |
| Gene ID | 142940 |
| Uniprot ID | TRUB1_HUMAN |
| Immunogen Region | 100-280 |
| Immunogen Sequence | PSPEWTKRKKQTLKIGHGGT LDSAARGVLVVGIGSGTKML TSMLSGSKRYTAIGELGKAT DTLDSTGRVTEEKPYDKITQ EDIEGILQKFTGNIMQVPPL YSALKKDGQRLSTLMKRGEV VEAKPARPVTVYSISLQKFQ PPFFTLDVECGGGFYIRSLV SDIGKELSSCANVLELTRTK Q |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TRUB1 (NP_631908.1). |
Additional Info
| Tissue Specificity | Highly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen. |
| Function | Pseudouridine synthase that catalyzes pseudouridylation of mRNAs and tRNAs. Mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3', harboring a stem-loop structure. Constitutes the major pseudouridine synthase acting on mRNAs. Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs. Promotes the processing of pri-let-7 microRNAs (pri-miRNAs) independently of its RNA pseudouridylate synthase activity. Acts by binding to the stem-loop structure on pri-let-7, preventing LIN28-binding (LIN28A and/or LIN28B), thereby enhancing the interaction between pri-let-7 and the microprocessor DGCR8, which mediates miRNA maturation. |
| Protein Name | Pseudouridylate Synthase Trub1Trub Pseudouridine Synthase Homolog 1Trna Pseudouridine 55 Synthase Trub1Psi55 Synthase Trub1 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytosolCatalyzes Pseudouridylation Of Mrnas In The Nucleus |
| Alternative Antibody Names | Anti-Pseudouridylate Synthase Trub1 antibodyAnti-Trub Pseudouridine Synthase Homolog 1 antibodyAnti-Trna Pseudouridine 55 Synthase Trub1 antibodyAnti-Psi55 Synthase Trub1 antibodyAnti-TRUB1 antibodyAnti-PUS4 antibody |
Information sourced from Uniprot.org