• Western blot analysis of extracts of mouse pancreas, using TRPC6 antibody (STJ110454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using TRPC6 Rabbit polyclonal antibody (STJ110454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat brain, using TRPC6 antibody (STJ110454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using TRPC6 Rabbit polyclonal antibody (STJ110454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using TRPC6 rabbit polyclonal antibody (STJ110454) at dilution of 1:150 (40x lens).
  • Western blot analysis of extracts of Rat brain, using TRPC6 antibody (STJ110454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded mouse kidney using TRPC6 rabbit polyclonal antibody (STJ110454) at dilution of 1:150 (40x lens).
  • Western blot analysis of extracts of Mouse pancreas, using TRPC6 antibody (STJ110454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRPC6 antibody (466-565) (STJ110454)

SKU:
STJ110454

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRPC6 (466-565) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRPC6
Gene ID: 7225
Uniprot ID: TRPC6_HUMAN
Immunogen Region: 466-565
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 466-565 of human TRPC6 (NP_004612.2).
Immunogen Sequence: EGTKLLPNETSTDNAKQLFR MKTSCFSWMEMLIISWVIGM IWAECKEIWTQGPKEYLFEL WNMLDFGMLAIFAASFIARF MAFWHASKAQSIIDANDTLK
Tissue Specificity Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
Post Translational Modifications Phosphorylated by FYN, leading to an increase of TRPC6 channel activity.
Function Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
Protein Name Short Transient Receptor Potential Channel 6
Trpc6
Transient Receptor Protein 6
Trp-6
Database Links Reactome: R-HSA-114508
Reactome: R-HSA-139853
Reactome: R-HSA-3295583
Reactome: R-HSA-418890
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Short Transient Receptor Potential Channel 6 antibody
Anti-Trpc6 antibody
Anti-Transient Receptor Protein 6 antibody
Anti-Trp-6 antibody
Anti-TRPC6 antibody
Anti-TRP6 antibody

Information sourced from Uniprot.org

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