| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRNT1 (135-434) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRNT1 |
| Gene ID: |
51095 |
| Uniprot ID: |
TRNT1_HUMAN |
| Immunogen Region: |
135-434 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 135-434 of human TRNT1 (NP_886552.2). |
| Immunogen Sequence: |
HAEVEFTTDWQKDAERRDLT INSMFLGFDGTLFDYFNGYE DLKNKKVRFVGHAKQRIQED YLRILRYFRFYGRIVDKPGD HDPETLEAIAENAKGLAGIS GERIWVELKKILVGNHVNHL IHLIYDLDVAPYIGLPANAS LEEFDKVSKNVDGFSPKPVT LLASLFKVQDDVTKLDLRLK IAKEEKNLGLFIVKNRKDLI KATDSSDPLKPYQDFIIDSR EPDATTRVCELLKYQGEHC |
| Function | Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Promotes tRNA repair and recycling downstream of the ribosome-associated quality control (RQC) pathway by mediating addition of the tRNA 3'-terminal CCA following cleavage by ANKZF1 and repair by ELAC1. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs and tRNA-like transcripts. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs beginning with GG are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Isoform 2: Adds 2 C residues (CC-) to the 3' terminus of tRNA molecules instead of a complete CCA end as isoform 1 does (in vitro). |
| Protein Name | Cca Trna Nucleotidyltransferase 1 - MitochondrialMitochondrial Trna Nucleotidyl Transferase - Cca-AddingMt Cca-Adding EnzymeMt Trna Cca-DiphosphorylaseMt Trna Cca-PyrophosphorylaseMt Trna Adenylyltransferase |
| Database Links | Reactome: R-HSA-6784531Reactome: R-HSA-6785470 |
| Cellular Localisation | MitochondrionCytoplasmNucleus |
| Alternative Antibody Names | Anti-Cca Trna Nucleotidyltransferase 1 - Mitochondrial antibodyAnti-Mitochondrial Trna Nucleotidyl Transferase - Cca-Adding antibodyAnti-Mt Cca-Adding Enzyme antibodyAnti-Mt Trna Cca-Diphosphorylase antibodyAnti-Mt Trna Cca-Pyrophosphorylase antibodyAnti-Mt Trna Adenylyltransferase antibodyAnti-TRNT1 antibodyAnti-CGI-47 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
