Anti-TRNT1 antibody (135-434) (STJ119742)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRNT1 (135-434) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRNT1 |
| Gene ID | 51095 |
| Uniprot ID | TRNT1_HUMAN |
| Immunogen Region | 135-434 |
| Immunogen Sequence | HAEVEFTTDWQKDAERRDLT INSMFLGFDGTLFDYFNGYE DLKNKKVRFVGHAKQRIQED YLRILRYFRFYGRIVDKPGD HDPETLEAIAENAKGLAGIS GERIWVELKKILVGNHVNHL IHLIYDLDVAPYIGLPANAS LEEFDKVSKNVDGFSPKPVT LLASLFKVQDDVTKLDLRLK IAKEEKNLGLFIVKNRKDLI KATDSSDPLKPYQDFIIDSR EPDATTRVCELLKYQGEHC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 135-434 of human TRNT1 (NP_886552.2). |
Additional Info
| Function | Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Promotes tRNA repair and recycling downstream of the ribosome-associated quality control (RQC) pathway by mediating addition of the tRNA 3'-terminal CCA following cleavage by ANKZF1 and repair by ELAC1. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs and tRNA-like transcripts. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs beginning with GG are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Isoform 2: Adds 2 C residues (CC-) to the 3' terminus of tRNA molecules instead of a complete CCA end as isoform 1 does (in vitro). |
| Protein Name | Cca Trna Nucleotidyltransferase 1 - MitochondrialMitochondrial Trna Nucleotidyl Transferase - Cca-AddingMt Cca-Adding EnzymeMt Trna Cca-DiphosphorylaseMt Trna Cca-PyrophosphorylaseMt Trna Adenylyltransferase |
| Database Links | Reactome: R-HSA-6784531Reactome: R-HSA-6785470 |
| Cellular Localisation | MitochondrionCytoplasmNucleus |
| Alternative Antibody Names | Anti-Cca Trna Nucleotidyltransferase 1 - Mitochondrial antibodyAnti-Mitochondrial Trna Nucleotidyl Transferase - Cca-Adding antibodyAnti-Mt Cca-Adding Enzyme antibodyAnti-Mt Trna Cca-Diphosphorylase antibodyAnti-Mt Trna Cca-Pyrophosphorylase antibodyAnti-Mt Trna Adenylyltransferase antibodyAnti-TRNT1 antibodyAnti-CGI-47 antibody |
Information sourced from Uniprot.org