• Western blot analysis of K-562, using TRMT2A/HTF9C rabbit monoclonal antibody (STJ11102674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of MCF7, using TRMT2A/HTF9C rabbit monoclonal antibody (STJ11102674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TRMT2A/HTF9C antibody (1-100) [S4MR] (STJ11102674)

SKU:
STJ11102674

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TRMT2A/HTF9C (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRMT2A
Gene ID: 27037
Uniprot ID: TRM2A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRMT2A/HTF9C (Q8IZ69).
Immunogen Sequence: MSENLDNEGPKPMESCGQES SSALSCPTVSVPPAAPAALE EVEKEGAGAATGPGPQPGLY SYIRDDLFTSEIFKLELQNV PRHASFSDVRRFLGRFGLQP
Function S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs. Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs. Also able to mediate the formation of 5-methyl-uridine in some mRNAs.
Protein Name Trna
Uracil-5--Methyltransferase Homolog A
Mrna
Uracil-5--Methyltransferase Trmt2a
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Trna antibody
Anti-Uracil-5--Methyltransferase Homolog A antibody
Anti-Mrna antibody
Anti-Uracil-5--Methyltransferase Trmt2a antibody
Anti-TRMT2A antibody

Information sourced from Uniprot.org

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