Anti-TRIM45 antibody (100-400) (STJ114612)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRIM45 (100-400) antibody for use in IF, ICC and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM45 |
| Gene ID | 80263 |
| Uniprot ID | TRI45_HUMAN |
| Immunogen Region | 100-400 |
| Immunogen Sequence | QVDLPMGGVKALTIDHLAVN DVMLESLRGEGQGLVCDLCN DREVEKRCQTCKANLCHFCC QAHRRQKKTTYHTMVDLKDL KGYSRIGKPILCPVHPAEEL RLFCEFCDRPVCQDCVVGEH REHPCDFTSNVIHKHGDSVW ELLKGTQPHVEALEEALAQI HIINSALQKRVEAVAADVRT FSEGYIKAIEEHRDKLLKQL EDIRAQKENSLQLQKAQLEQ LLADMRTGVEFTEHLLTSG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM45 (NP_001139107.1). |
Additional Info
| Tissue Specificity | Expressed in skeletal muscle, brain, heart and pancreas. |
| Function | E3 ubiquitin-protein ligase that plays a role in the regulation of inflammatory response. Mechanistically, mediates the 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, leading to its degradation via the proteasomal pathway and inhibition of the TLR-mediated inflammatory immune response. May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim45Ring Finger Protein 99 |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim45 antibodyAnti-Ring Finger Protein 99 antibodyAnti-TRIM45 antibodyAnti-RNF99 antibody |
Information sourced from Uniprot.org