Anti-TRIM32 antibody (STJA0006910)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human TRIM32
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase TRIM32 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM32 |
| Gene ID | 22954 |
| Uniprot ID | TRI32_HUMAN |
| Immunogen | Synthesized peptide derived from human TRIM32 |
| Specificity | This antibody detects endogenous levels of TRIM32 at Human, Mouse |
Additional Info
| Post Translational Modifications | Ubiquitinated. Phosphorylation at Ser-55 by CHEK2 under oxidative stress, activates the E3 ligase activity and promotes ATG7 ubiquitination leading to positive regulation of the autophagosme assembly. |
| Function | E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy. Plays a role in virus-triggered induction of IFN-beta and TNF-alpha by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1. Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages. Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner. Plays an essential role in oxidative stress induced cell death by inducing loss of transmembrane potential and enhancing mitochondrial reactive oxygen species (ROS) production during oxidative stress conditions. Ubiquitinates XIAP and targets it for proteasomal degradation. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha. Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation. Positively regulates dendritic branching by promoting ubiquitination and subsequent degradation of the epigenetic factor CDYL. Under metabolic stress and phosphorylation by CHK2, mediates 'Lys-63'-linked ubiquitination of ATG7 at 'Lys-45' to initiate autophagy. (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim3272 Kda Tat-Interacting ProteinRing-Type E3 Ubiquitin Transferase Trim32Tripartite Motif-Containing Protein 32Zinc Finger Protein Ht2a |
| Database Links | Reactome: R-HSA-3134975Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmMitochondrionEndoplasmic ReticulumLocalized In Cytoplasmic BodiesOften Located Around The Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim32 antibodyAnti-72 Kda Tat-Interacting Protein antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim32 antibodyAnti-Tripartite Motif-Containing Protein 32 antibodyAnti-Zinc Finger Protein Ht2a antibodyAnti-TRIM32 antibodyAnti-HT2A antibody |
Information sourced from Uniprot.org