| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC/IHC |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TRIM29/ATDC (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 3 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TRIM29 |
| Gene ID: | 23650 |
| Uniprot ID: | TRI29_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_036233.2; NP_001317311.1 |
| Immunogen Sequence: | EKDRIKSFTTNE |
| Post Translational Modifications | Constitutively phosphorylated by PKC on serine/threonine in A431 cells. |
| Function | Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation. |
| Protein Name | Tripartite Motif-Containing Protein 29Ataxia Telangiectasia Group D-Associated Protein |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmLysosomeColocalizes With Intermediate Filaments |
| Alternative Antibody Names | Anti-Tripartite Motif-Containing Protein 29 antibodyAnti-Ataxia Telangiectasia Group D-Associated Protein antibodyAnti-TRIM29 antibodyAnti-ATDC antibody |
Information sourced from Uniprot.org