• STJ72127 (1µg/ml) staining of Peripheral Blood Mononucleocyte (A) and (0. 1 ug/ml) HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72127 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic and Intermediate filament staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ72127 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ72127 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-TRIM29/ATDC antibody (Internal) (STJ72127)

SKU:
STJ72127-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TRIM29/ATDC (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRIM29
Gene ID: 23650
Uniprot ID: TRI29_HUMAN
Immunogen Region: Internal
Accession Number: NP_036233.2; NP_001317311.1
Immunogen Sequence: EKDRIKSFTTNE
Post Translational Modifications Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
Function Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.
Protein Name Tripartite Motif-Containing Protein 29
Ataxia Telangiectasia Group D-Associated Protein
Database Links Reactome: R-HSA-877300
Cellular Localisation Cytoplasm
Lysosome
Colocalizes With Intermediate Filaments
Alternative Antibody Names Anti-Tripartite Motif-Containing Protein 29 antibody
Anti-Ataxia Telangiectasia Group D-Associated Protein antibody
Anti-TRIM29 antibody
Anti-ATDC antibody

Information sourced from Uniprot.org

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