• Western blot analysis of various lysates, using TRIM25 rabbit polyclonal antibody (STJ114804) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using TRIM25 rabbit polyclonal antibody (STJ114804) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse intestin using TRIM25 rabbit polyclonal antibody (STJ114804) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using TRIM25 rabbit polyclonal antibody (STJ114804) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A-431 cells using TRIM25 rabbit polyclonal antibody (STJ114804) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using TRIM25 rabbit polyclonal antibody (STJ114804) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g TRIM25 antibody (STJ114804). Western blot was performed from the immunoprecipitate using TRIM25 antibody (STJ114804) at a dilution of 1:1000.

Anti-TRIM25 antibody (190-630) (STJ114804)

SKU:
STJ114804

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM25 (190-630) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM25
Gene ID: 7706
Uniprot ID: TRI25_HUMAN
Immunogen Region: 190-630
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-630 of human TRIM25 (NP_005073.2).
Immunogen Sequence: SLSQASADLEATLRHKLTVM YSQINGASRALDDVRNRQQD VRMTANRKVEQLQQEYTEMK ALLDASETTSTRKIKEEEKR VNSKFDTIYQILLKKKSEIQ TLKEEIEQSLTKRDEFEFLE KASKLRGISTKPVYIPEVEL NHKLIKGIHQSTIDLKNELK QCIGRLQEPTPSSGDPGEHD PASTHKSTRP
Tissue Specificity Expressed in breast tumors (at protein level). Ubiquitous.
Post Translational Modifications Auto-ISGylated. (Microbial infection) Autoubiquitinated.promoted by Epstein-Barr virus protein BPLF1.
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks. Plays an essential role in the antiviral activity of ZAP/ZC3HAV1.an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA. Mediates also the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability.
Protein Name E3 Ubiquitin/Isg15 Ligase Trim25
Estrogen-Responsive Finger Protein
Ring Finger Protein 147
Ring-Type E3 Ubiquitin Transferase
Ring-Type E3 Ubiquitin Transferase Trim25
Tripartite Motif-Containing Protein 25
Ubiquitin/Isg15-Conjugating Enzyme Trim25
Zinc Finger Protein 147
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-168928
Reactome: R-HSA-5656169
Reactome: R-HSA-5689896
Reactome: R-HSA-877300
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-933542
Reactome: R-HSA-933543
Reactome: R-HSA-936440
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Stress Granule
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin/Isg15 Ligase Trim25 antibody
Anti-Estrogen-Responsive Finger Protein antibody
Anti-Ring Finger Protein 147 antibody
Anti-Ring-Type E3 Ubiquitin Transferase antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim25 antibody
Anti-Tripartite Motif-Containing Protein 25 antibody
Anti-Ubiquitin/Isg15-Conjugating Enzyme Trim25 antibody
Anti-Zinc Finger Protein 147 antibody
Anti-TRIM25 antibody
Anti-EFP antibody
Anti-RNF147 antibody
Anti-ZNF147 antibody

Information sourced from Uniprot.org

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