| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM25 |
| Gene ID: | 7706 |
| Uniprot ID: | TRI25_HUMAN |
| Immunogen Region: | 190-630 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-379 of human TRIM25 (NP_005073.2). |
| Immunogen Sequence: | SLSQASADLEATLRHKLTVM YSQINGASRALDDVRNRQQD VRMTANRKVEQLQQEYTEMK ALLDASETTSTRKIKEEEKR VNSKFDTIYQILLKKKSEIQ TLKEEIEQSLTKRDEFEFLE KASKLRGISTKPVYIPEVEL NHKLIKGIHQSTIDLKNELK QCIGRLQEPTPSSGDPGEHD PASTHKSTRP |
| Tissue Specificity | Expressed in breast tumors (at protein level). Ubiquitous. |
| Post Translational Modifications | Auto-ISGylated. (Microbial infection) Autoubiquitinated.promoted by Epstein-Barr virus protein BPLF1. |
| Function | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks. Plays an essential role in the antiviral activity of ZAP/ZC3HAV1.an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA. Also mediates the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability. |
| Protein Name | E3 Ubiquitin/Isg15 Ligase Trim25Estrogen-Responsive Finger ProteinRing Finger Protein 147Ring-Type E3 Ubiquitin TransferaseRing-Type E3 Ubiquitin Transferase Trim25Tripartite Motif-Containing Protein 25Ubiquitin/Isg15-Conjugating Enzyme Trim25Zinc Finger Protein 147 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168928Reactome: R-HSA-5656169Reactome: R-HSA-5689896Reactome: R-HSA-877300Reactome: R-HSA-918233Reactome: R-HSA-933541Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-936440Reactome: R-HSA-9692916Reactome: R-HSA-9705671Reactome: R-HSA-9833109Reactome: R-HSA-9833110Reactome: R-HSA-9833482Reactome: R-HSA-9909505 |
| Cellular Localisation | CytoplasmStress GranuleNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin/Isg15 Ligase Trim25 antibodyAnti-Estrogen-Responsive Finger Protein antibodyAnti-Ring Finger Protein 147 antibodyAnti-Ring-Type E3 Ubiquitin Transferase antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim25 antibodyAnti-Tripartite Motif-Containing Protein 25 antibodyAnti-Ubiquitin/Isg15-Conjugating Enzyme Trim25 antibodyAnti-Zinc Finger Protein 147 antibodyAnti-TRIM25 antibodyAnti-EFP antibodyAnti-RNF147 antibodyAnti-ZNF147 antibody |
Information sourced from Uniprot.org
