• Western blot analysis of extracts of A-549 cells, using TPX2 antibody (STJ11100283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using TPX2 rabbit polyclonal antibody (STJ11100283) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using TPX2 rabbit polyclonal antibody (STJ11100283) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using TPX2 rabbit polyclonal antibody (STJ11100283) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using TPX2 rabbit polyclonal antibody (STJ11100283) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Anti-TPX2 antibody (STJ11100283)

SKU:
STJ11100283

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-TPX2 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: TPX2
Gene ID: 22974
Uniprot ID: TPX2_HUMAN
Immunogen: Recombinant protein of human TPX2.
Tissue Specificity Expressed in lung carcinoma cell lines but not in normal lung tissues.
Function Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
Protein Name Targeting Protein For Xklp2
Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2
Dil-2
Hepatocellular Carcinoma-Associated Antigen 519
Hepatocellular Carcinoma-Associated Antigen 90
Protein Fls353
Restricted Expression Proliferation-Associated Protein 100
P100
Database Links Reactome: R-HSA-6804756
Reactome: R-HSA-8854518
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Spindle Pole
During Mitosis It Is Strictly Associated With The Spindle Pole And With The Mitotic Spindle
Whereas During S And G2
It Is Diffusely Distributed Throughout The Nucleus
Is Released From The Nucleus In Apoptotic Cells And Is Detected On Apoptotic Microtubules
Alternative Antibody Names Anti-Targeting Protein For Xklp2 antibody
Anti-Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2 antibody
Anti-Dil-2 antibody
Anti-Hepatocellular Carcinoma-Associated Antigen 519 antibody
Anti-Hepatocellular Carcinoma-Associated Antigen 90 antibody
Anti-Protein Fls353 antibody
Anti-Restricted Expression Proliferation-Associated Protein 100 antibody
Anti-P100 antibody
Anti-TPX2 antibody
Anti-C20orf1 antibody
Anti-C20orf2 antibody
Anti-DIL2 antibody
Anti-HCA519 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance