||WB, IHC, IF
||FOR RESEARCH USE ONLY (RUO).
||Rabbit polyclonal antibody anti-TPX2 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
||PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
||WB 1:500-1:2000IHC 1:50-1:200IF 1:50-1:200
||Store in a freezer at-20°C and avoid freeze-thaw cycles.
||Recombinant protein of human TPX2.
| Tissue Specificity || Expressed in lung carcinoma cell lines but not in normal lung tissues. |
| Function || Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation. |
| Protein Name || Targeting Protein For Xklp2Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2Dil-2Hepatocellular Carcinoma-Associated Antigen 519Hepatocellular Carcinoma-Associated Antigen 90Protein Fls353Restricted Expression Proliferation-Associated Protein 100P100 |
| Database Links || Reactome: R-HSA-6804756Reactome: R-HSA-8854518 |
| Cellular Localisation || NucleusCytoplasmCytoskeletonSpindleSpindle PoleDuring Mitosis It Is Strictly Associated With The Spindle Pole And With The Mitotic SpindleWhereas During S And G2It Is Diffusely Distributed Throughout The NucleusIs Released From The Nucleus In Apoptotic Cells And Is Detected On Apoptotic Microtubules |
| Alternative Antibody Names || Anti-Targeting Protein For Xklp2 antibodyAnti-Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2 antibodyAnti-Dil-2 antibodyAnti-Hepatocellular Carcinoma-Associated Antigen 519 antibodyAnti-Hepatocellular Carcinoma-Associated Antigen 90 antibodyAnti-Protein Fls353 antibodyAnti-Restricted Expression Proliferation-Associated Protein 100 antibodyAnti-P100 antibodyAnti-TPX2 antibodyAnti-C20orf1 antibodyAnti-C20orf2 antibodyAnti-DIL2 antibodyAnti-HCA519 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance