Anti-TPX2 antibody (1-250) (STJ11100283)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TPX2 (1-250) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TPX2 |
| Gene ID | 22974 |
| Uniprot ID | TPX2_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MSQVKSSYSYDAPSDFINFS SLDDEGDTQNIDSWFEEKAN LENKLLGKNGTGGLFQGKTP LRKANLQQAIVTPLKPVDNT YYKEAEKENLVEQSIPSNAC SSLEVEAAISRKTPAQPQRR SLRLSAQKDLEQKEKHHVKM KAKRCATPVIIDEILPSKKM KVSNNKKKPEEEGSAHQDTA EKNASSPEKAKGRHTVPCMP PAKQKFLKSTEEQELEKSMK MQQEVVEMRKKNEEFKKLA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TPX2 (NP_036244.2). |
Additional Info
| Tissue Specificity | Expressed in lung carcinoma cell lines but not in normal lung tissues. |
| Function | Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation. |
| Protein Name | Targeting Protein For Xklp2Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2Dil-2Hepatocellular Carcinoma-Associated Antigen 519Hepatocellular Carcinoma-Associated Antigen 90Protein Fls353Restricted Expression Proliferation-Associated Protein 100P100 |
| Database Links | Reactome: R-HSA-6804756Reactome: R-HSA-8854518 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindleSpindle PoleDuring Mitosis It Is Strictly Associated With The Spindle Pole And With The Mitotic SpindleWhereas During S And G2It Is Diffusely Distributed Throughout The NucleusIs Released From The Nucleus In Apoptotic Cells And Is Detected On Apoptotic Microtubules |
| Alternative Antibody Names | Anti-Targeting Protein For Xklp2 antibodyAnti-Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2 antibodyAnti-Dil-2 antibodyAnti-Hepatocellular Carcinoma-Associated Antigen 519 antibodyAnti-Hepatocellular Carcinoma-Associated Antigen 90 antibodyAnti-Protein Fls353 antibodyAnti-Restricted Expression Proliferation-Associated Protein 100 antibodyAnti-P100 antibodyAnti-TPX2 antibodyAnti-C20orf1 antibodyAnti-C20orf2 antibodyAnti-DIL2 antibodyAnti-HCA519 antibody |
Information sourced from Uniprot.org