Anti-TOP2B antibody (1-50 aa) (STJ96068)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TOP2B at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-DNA topoisomerase 2-beta (1-50 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TOP2B |
| Gene ID | 7155 |
| Uniprot ID | TOP2B_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TOP2B at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | Topo II Beta Polyclonal Antibody detects endogenous levels of Topo II Beta protein. |
Additional Info
| Post Translational Modifications | (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation. |
| Protein Name | Dna Topoisomerase 2-BetaDna Topoisomerase Ii - Beta Isozyme |
| Database Links | Reactome: R-HSA-4615885 |
| Cellular Localisation | NucleusNucleolusNucleoplasm |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Beta antibodyAnti-Dna Topoisomerase Ii - Beta Isozyme antibodyAnti-TOP2B antibody |
Information sourced from Uniprot.org