| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TOM1 (314-493) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TOM1 |
| Gene ID: |
10043 |
| Uniprot ID: |
TOM1_HUMAN |
| Immunogen Region: |
314-493 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 314-493 of human TOM1 (NP_001129204.1). |
| Immunogen Sequence: |
SEAEPAADLIDMGPDPAATG NLSSQLAGMNLGSSSVRAGL QSLEASGRLEDEFDMFALTR GSSLADQRKEVKYEAPQATD GLAGALDARQQSTGAIPVTQ ACLMEDIEQWLSTDVGNDAE EPKGVTSEGKFDKFLEERAK AADRLPNLSSPSAEGPPGPP SGPAPRKKTQEKDDDMLFAL |
| Tissue Specificity | Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. |
| Post Translational Modifications | Monoubiquitinated. |
| Function | Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways. Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures. Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes. MYO6 links TOM1 with autophagy receptors, such as TAX1BP1.CALCOCO2/NDP52 and OPTN. Binds to polyubiquitinated proteins via its GAT domain. In a complex with TOLLIP, recruits ubiquitin-conjugated proteins onto early endosomes. The Tom1-Tollip complex may regulate endosomal trafficking by linking polyubiquitinated proteins to clathrin. Mediates clathrin recruitment to early endosomes by ZFYVE16. Modulates binding of TOLLIP to phosphatidylinositol 3-phosphate (PtdIns(3)P) via binding competition.the association with TOLLIP may favor the release of TOLLIP from endosomal membranes, allowing TOLLIP to commit to cargo trafficking. Acts as a phosphatidylinositol 5-phosphate (PtdIns(5)P) effector by binding to PtdIns(5)P, thereby regulating endosomal maturation. PtdIns(5)P-dependent recruitment to signaling endosomes may block endosomal maturation. Also inhibits Toll-like receptor (TLR) signaling and participates in immune receptor recycling. |
| Protein Name | Target Of Myb1 Membrane Trafficking ProteinTarget Of Myb Protein 1 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmEndosome MembranePeripheral Membrane ProteinEarly Endosome MembraneLocalized To Endo/Exosomal VesiclesEnriched On Signaling EndosomesRecruited To Early Endosomes By Tollip And By Ptdins(5)P |
| Alternative Antibody Names | Anti-Target Of Myb1 Membrane Trafficking Protein antibodyAnti-Target Of Myb Protein 1 antibodyAnti-TOM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
