• Immunohistochemical analysis of paraffin-embedded human-Lymph-nodes, antibody was diluted at 1:100
  • Western blot analysis of lysate from 293 cells, using TNFSF11 Antibody.
  • Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-Lymph-nodes, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
  • Western blot analysis of 293 cells using RANKL Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, RANKL Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, RANKL Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, RANKL Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-TNFSF11 antibody (268-317 aa) (STJ96769)

SKU:
STJ96769

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tumor necrosis factor ligand superfamily member 11 (268-317 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFSF11
Gene ID: 8600
Uniprot ID: TNF11_HUMAN
Immunogen Region: 268-317 aa
Specificity: RANKL Polyclonal Antibody detects endogenous levels of RANKL protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human TNFSF11 at the amino acid range 268-317
Post Translational Modifications The soluble form of isoform 1 derives from the membrane form by proteolytic processing. The cleavage may be catalyzed by ADAM17.
Function Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 11
Osteoclast Differentiation Factor
Odf
Osteoprotegerin Ligand
Opgl
Receptor Activator Of Nuclear Factor Kappa-B Ligand
Rankl
Tnf-Related Activation-Induced Cytokine
Trance
Cd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5669034
Reactome: R-HSA-5676594
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type Ii Membrane Protein
Isoform 3: Cell Membrane
Isoform 2: Cytoplasm
Tumor Necrosis Factor Ligand Superfamily Member 11
Soluble Form: Secreted
Alternative Antibody Names Anti-Tumor Necrosis Factor Ligand Superfamily Member 11 antibody
Anti-Osteoclast Differentiation Factor antibody
Anti-Odf antibody
Anti-Osteoprotegerin Ligand antibody
Anti-Opgl antibody
Anti-Receptor Activator Of Nuclear Factor Kappa-B Ligand antibody
Anti-Rankl antibody
Anti-Tnf-Related Activation-Induced Cytokine antibody
Anti-Trance antibody
Anti-Cd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form antibody
Anti-TNFSF11 antibody
Anti-OPGL antibody
Anti-RANKL antibody
Anti-TRANCE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance