• Western blot analysis of extracts of THP-1 cells, using (STJ25899) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TNFSF10 antibody (1-281) (STJ25899)

SKU:
STJ25899

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNFSF10 (1-281) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFSF10
Gene ID: 8743
Uniprot ID: TNF10_HUMAN
Immunogen Region: 1-281
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human TNFSF10 (NP_003801.1).
Immunogen Sequence: MAMMEVQGGPSLGQTCVLIV IFTVLLQSLCVAVTYVYFTN ELKQMQDKYSKSGIACFLKE DDSYWDPNDEESMNSPCWQV KWQLRQLVRKMILRTSEETI
Tissue Specificity Widespread.most predominant in spleen, lung and prostate.
Post Translational Modifications Tyrosine phosphorylated by PKDCC/VLK.
Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 10
Apo-2 Ligand
Apo-2l
Tnf-Related Apoptosis-Inducing Ligand
Protein Trail
Cd Antigen Cd253
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-69416
Reactome: R-HSA-75158
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Exists Both As Membrane-Bound And Soluble Form
Alternative Antibody Names Anti-Tumor Necrosis Factor Ligand Superfamily Member 10 antibody
Anti-Apo-2 Ligand antibody
Anti-Apo-2l antibody
Anti-Tnf-Related Apoptosis-Inducing Ligand antibody
Anti-Protein Trail antibody
Anti-Cd Antigen Cd253 antibody
Anti-TNFSF10 antibody
Anti-APO2L antibody
Anti-TRAIL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance