| Host: |
Goat |
| Applications: |
Pep-ELISA/IF/FC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-TMP21/TMED10 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IF-Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. 10µg/mlELISA-antibody detection limit dilution 1:1000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
TMED10 |
| Gene ID: |
10972 |
| Uniprot ID: |
TMEDA_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_006818.3 |
| Immunogen Sequence: |
YLRRFFKAKKLIE |
| Protein Name | Transmembrane Emp24 Domain-Containing Protein 10Protein Tmed1021 Kda Transmembrane-Trafficking ProteinS31i125S31iii125Tmp-21-ITransmembrane Protein Tmp21P23P24 Family Protein Delta-1P24delta1P24delta |
| Database Links | Reactome: R-HSA-204005Reactome: R-HSA-5694530Reactome: R-HSA-6807878Reactome: R-HSA-6811434 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneGolgi Apparatus MembraneGolgi ApparatusCis-Golgi Network MembraneTrans-Golgi Network MembraneCytoplasmic VesicleSecretory Vesicle MembraneCell MembraneMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Transmembrane Emp24 Domain-Containing Protein 10 antibodyAnti-Protein Tmed10 antibodyAnti-21 Kda Transmembrane-Trafficking Protein antibodyAnti-S31i125 antibodyAnti-S31iii125 antibodyAnti-Tmp-21-I antibodyAnti-Transmembrane Protein Tmp21 antibodyAnti-P23 antibodyAnti-P24 Family Protein Delta-1 antibodyAnti-P24delta1 antibodyAnti-P24delta antibodyAnti-TMED10 antibodyAnti-TMP21 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit polyclonal antibody (STJ11100063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89133/357582/STJ11100063_1__14749.1713121714.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit polyclonal antibody (STJ11100063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
