Anti-TMEM175 antibody (STJA0007121)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human TMEM175
General Information
| Short Description | Rabbit polyclonal anti-Endosomal/lysosomal proton channel TMEM175 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TMEM175 |
| Gene ID | 84286 |
| Uniprot ID | TM175_HUMAN |
| Immunogen | Synthesized peptide derived from human TMEM175 |
| Specificity | This antibody detects endogenous levels of TMEM175 at Human, Mouse, Rat |
Additional Info
| Function | Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH. Activated at low pH (under pH 4.6) by luminal side protons: selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis. Regulation of lumenal pH stability is required for autophagosome-lysosome fusion. Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes. Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors. The lysoK(GF) complex is composed of TMEM175 and AKT (AKT1, AKT2 or AKT3), a major target of growth factor receptors: in the complex, TMEM175 channel is opened by conformational changes by AKT, leading to its activation. The lysoK(GF) complex is required to protect neurons against stress-induced damage. |
| Protein Name | Endosomal/Lysosomal Proton Channel Tmem175Potassium Channel Tmem175Transmembrane Protein 175Htmem175 |
| Database Links | |
| Cellular Localisation | Endosome MembraneMulti-Pass Membrane ProteinLysosome Membrane |
| Alternative Antibody Names | Anti-Endosomal/Lysosomal Proton Channel Tmem175 antibodyAnti-Potassium Channel Tmem175 antibodyAnti-Transmembrane Protein 175 antibodyAnti-Htmem175 antibodyAnti-TMEM175 antibody |
Information sourced from Uniprot.org