• Immunofluorescence analysis of A549 cells using TIMP4 Rabbit polyclonal antibody (STJ28499) at a dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using TIMP4 Rabbit polyclonal antibody (STJ28499) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-TIMP4 antibody (30-224) (STJ28499)

SKU:
STJ28499

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMP4 (30-224) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMP4
Gene ID: 7079
Uniprot ID: TIMP4_HUMAN
Immunogen Region: 30-224
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-224 of human TIMP4 (NP_003247.1).
Immunogen Sequence: CSCAPAHPQQHICHSALVIR AKISSEKVVPASADPADTEK MLRYEIKQIKMFKGFEKVKD VQYIYTPFDSSLCGVKLEAN SQKQYLLTGQVLSDGKVFIH LCNYIEPWEDLSLVQRESLN HHYHLNCGCQITTCYTVPCT ISAPNECLWTDWLLERKLYG YQAQHYVCMKHVDGTCSWYR GHLPLRKEFVDIVQP
Tissue Specificity Abundant in heart and present at low levels in many other tissues.
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Protein Name Metalloproteinase Inhibitor 4
Tissue Inhibitor Of Metalloproteinases 4
Timp-4
Cellular Localisation Secreted
Alternative Antibody Names Anti-Metalloproteinase Inhibitor 4 antibody
Anti-Tissue Inhibitor Of Metalloproteinases 4 antibody
Anti-Timp-4 antibody
Anti-TIMP4 antibody

Information sourced from Uniprot.org

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