• Immunofluorescence analysis of U-2 OS cells using TIMM8B Rabbit polyclonal antibody (STJ118259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using TIMM8B Rabbit polyclonal antibody (STJ118259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TIMM8B in paraffin-embedded mouse heart using TIMM8B Rabbit polyclonal antibody (STJ118259) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TIMM8B in paraffin-embedded human breast cancer using TIMM8B Rabbit polyclonal antibody (STJ118259) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TIMM8B in paraffin-embedded rat brain using TIMM8B Rabbit polyclonal antibody (STJ118259) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TIMM8B Rabbit polyclonal antibody (STJ118259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TIMM8B antibody (16-98) (STJ118259)

SKU:
STJ118259

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMM8B (16-98) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM8B
Gene ID: 26521
Uniprot ID: TIM8B_HUMAN
Immunogen Region: 16-98
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 16-98 of human TIMM8B (NP_036591.1).
Immunogen Sequence: MAELGEADEAELQRLVAAEQ QKAQFTAQVHHFMELCWDKC VEKPGNRLDSRTENCLSSCV DRFIDTTLAITSRFAQIVQK GGQ
Tissue Specificity Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
Function Probable mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim8 B
Ddp-Like Protein
Deafness Dystonia Protein 2
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Intermembrane Side
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim8 B antibody
Anti-Ddp-Like Protein antibody
Anti-Deafness Dystonia Protein 2 antibody
Anti-TIMM8B antibody
Anti-DDP2 antibody
Anti-DDPL antibody
Anti-TIM8B antibody

Information sourced from Uniprot.org

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