Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to TGN (STJ13100506) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-TGN38 antibody (STJ13100506)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from mouse TGN38 conjugated to blue carrier protein was used as the antigen.
STJ13100506-500
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General Information

Short DescriptionRabbit polyclonal anti-TGN38 for use in IHC and WB in Rat, Mouse, Human and Marmoset samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB
HostRabbit
ReactivityRat/Mouse/Human/Marmoset
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
PurificationIgG purified
Dilution RangeIHC, WB. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
FormulationLyophilised
Storage InstructionMaintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Target Information

Gene SymbolTgoln2
Gene ID22135
Uniprot IDTGON2_MOUSE
ImmunogenA synthetic peptide from mouse TGN38 conjugated to blue carrier protein was used as the antigen.
SpecificitySpecific for TGN38 (or TGN46, TGN48, TGN51 in human).

Additional Info

Function May be involved in regulating membrane traffic to and from trans-Golgi network.
Protein Name Trans-Golgi Network Integral Membrane Protein 2
Tgn38b
Database Links
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Primarily In Trans-Golgi Network
Cycles Between The Trans-Golgi Network And The Cell Surface Returning Via Endosomes
Alternative Antibody Names Anti-Trans-Golgi Network Integral Membrane Protein 2 antibody
Anti-Tgn38b antibody
Anti-Tgoln2 antibody
Anti-Ttgn2 antibody

Information sourced from Uniprot.org

Citations

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