Immunohistochemistry analysis of TGM5 in paraffin-embedded mouse heart using TGM5 Rabbit polyclonal antibody (STJ29119) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TGM5 antibody (461-720) (STJ29119)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ29119
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General Information

Short DescriptionRabbit polyclonal TGM5 (461-720) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:2000-1:8000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTGM5
Gene ID9333
Uniprot IDTGM5_HUMAN
Immunogen Region461-720
Immunogen SequenceLQKLKARSFHGSQRGAELQP SRPTSLSQDSPRSLHTPSLR PSDVVQVSLKFKLLDPPNMG QDICFVLLALNMSSQFKDLK VNLSAQSLLHDGSPLSPFWQ DTAFITLSPKEAKTYPCKIS YSQYSQYLSTDKLIRISALG EEKSSPEKILVNKIITLSYP SITINVLGAAVVNQPLSIQV IFSNPLSEQVEDCVLTVEGS GLFKKQQKVFLGVLKPQHQA SIILETVPFKSGQRQIQAN
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2).

Additional Info

Tissue Specificity Expressed in foreskin keratinocytes.
Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes.
Protein Name Protein-Glutamine Gamma-Glutamyltransferase 5
Transglutaminase X
Tg(X
Tgx
Tgase X
Transglutaminase-5
Tgase-5
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative Antibody Names Anti-Protein-Glutamine Gamma-Glutamyltransferase 5 antibody
Anti-Transglutaminase X antibody
Anti-Tg(X antibody
Anti-Tgx antibody
Anti-Tgase X antibody
Anti-Transglutaminase-5 antibody
Anti-Tgase-5 antibody
Anti-TGM5 antibody
Anti-TGMX antibody

Information sourced from Uniprot.org

Citations

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