• STJ71197 (0. 03µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ71197 (0. 1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ71197 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-TGM2 antibody (Internal) (STJ71197)

SKU:
STJ71197-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TGM2 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.1µg/ml
IHC-5-10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TGM2
Gene ID: 7052
Uniprot ID: TGM2_HUMAN
Immunogen Region: Internal
Accession Number: NP_004604.2
Specificity: This antibody is expected to recognise isoform a (NP_004604.2) only.
Immunogen Sequence: NFESDKLKAVKGFR
Function Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP.inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively. Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases. Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3. Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription. Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system. Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2. Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate. Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet. May also act as an isopeptidase cleaving the previously formed cross-links. Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism. Isoform 2: Has cytotoxic activity: is able to induce apoptosis independently of its acyltransferase activity.
Protein Name Protein-Glutamine Gamma-Glutamyltransferase 2
Erythrocyte Transglutaminase
Heart G Alpha(H
Hhg Alpha(H
Isopeptidase Tgm2
Protein G Alpha(H
G(H
Protein-Glutamine Deamidase Tgm2
Protein-Glutamine Dopaminyltransferase Tgm2
Protein-Glutamine Histaminyltransferase Tgm2
Protein-Glutamine Noradrenalinyltransferase Tgm2
Protein-Glutamine Serotonyltransferase Tgm2
Tissue Transglutaminase
Ttg
Ttgase
Transglutaminase C
Tg(C
Tgc
Tgase C
Transglutaminase H
Tgase H
Transglutaminase Ii
Tgase Ii
Transglutaminase-2
Tg2
Tgase-2
Htg2
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Chromosome
Secreted
Extracellular Space
Extracellular Matrix
Cell Membrane
Mitochondrion
Mainly Localizes To The Cytosol
Present At Much Lower Level In The Nucleus And Chromatin
Also Secreted Via A Non-Classical Secretion Pathway To The Extracellular Matrix
Isoform 2: Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Protein-Glutamine Gamma-Glutamyltransferase 2 antibody
Anti-Erythrocyte Transglutaminase antibody
Anti-Heart G Alpha(H antibody
Anti-Hhg Alpha(H antibody
Anti-Isopeptidase Tgm2 antibody
Anti-Protein G Alpha(H antibody
Anti-G(H antibody
Anti-Protein-Glutamine Deamidase Tgm2 antibody
Anti-Protein-Glutamine Dopaminyltransferase Tgm2 antibody
Anti-Protein-Glutamine Histaminyltransferase Tgm2 antibody
Anti-Protein-Glutamine Noradrenalinyltransferase Tgm2 antibody
Anti-Protein-Glutamine Serotonyltransferase Tgm2 antibody
Anti-Tissue Transglutaminase antibody
Anti-Ttg antibody
Anti-Ttgase antibody
Anti-Transglutaminase C antibody
Anti-Tg(C antibody
Anti-Tgc antibody
Anti-Tgase C antibody
Anti-Transglutaminase H antibody
Anti-Tgase H antibody
Anti-Transglutaminase Ii antibody
Anti-Tgase Ii antibody
Anti-Transglutaminase-2 antibody
Anti-Tg2 antibody
Anti-Tgase-2 antibody
Anti-Htg2 antibody
Anti-TGM2 antibody

Information sourced from Uniprot.org

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