• Immunohistochemistry analysis of TGF beta induced (TGFBI) in paraffin-embedded human esophageal using TGF beta induced TGF beta induced (TGFBI) Rabbit monoclonal antibody (STJ11101820) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TGF beta induced TGF beta induced (TGFBI) Rabbit monoclonal antibody (STJ11101820) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using TGF beta induced TGF beta induced (TGFBI) Rabbit monoclonal antibody (STJ11101820) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TGFBI antibody (50-150) [S0MR] (STJ11101820)

SKU:
STJ11101820

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TGFBI (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000 IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TGFBI
Gene ID: 7045
Uniprot ID: BGH3_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TGF beta induced (TGFBI) (Q15582).
Immunogen Sequence: AVQKVIGTNRKYFTNCKQWY QRKICGKSTVISYECCPGYE KVPGEKGCPAALPLSNLYET LGVVGSTTTQLYTDRTEKLR PEMEGPGSFTIFAPSNEAWA S
Tissue Specificity Highly expressed in the corneal epithelium. Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.
Function Plays a role in cell adhesion. May play a role in cell-collagen interactions.
Protein Name Transforming Growth Factor-Beta-Induced Protein Ig-H3
Beta Ig-H3
Kerato-Epithelin
Rgd-Containing Collagen-Associated Protein
Rgd-Cap
Database Links Reactome: R-HSA-977225
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
May Be Associated Both With Microfibrils And With The Cell Surface
Alternative Antibody Names Anti-Transforming Growth Factor-Beta-Induced Protein Ig-H3 antibody
Anti-Beta Ig-H3 antibody
Anti-Kerato-Epithelin antibody
Anti-Rgd-Containing Collagen-Associated Protein antibody
Anti-Rgd-Cap antibody
Anti-TGFBI antibody
Anti-BIGH3 antibody

Information sourced from Uniprot.org

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