• Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and TFAP4 knockout (KO) HeLa cells, using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60S.
  • Western blot analysis of various lysates using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-TFAP4 antibody (200-338) (STJ119138)

SKU:
STJ119138

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TFAP4 (200-338) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFAP4
Gene ID: 7023
Uniprot ID: TFAP4_HUMAN
Immunogen Region: 200-338
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-338 of human TFAP4 (NP_003214.1).
Immunogen Sequence: QQEQVRLLHQEKLEREQQQL RTQLLPPPAPTHHPTVIVPA PPPPPSHHINVVTMGPSSVI NSVSTSRQNLDTIVQAIQHI EGTQEKQELEEEQRRAVIVK PVRSCPEAPTSDTASDSEAS DSDAMDQSREEPSGDGELP
Function Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'.
Protein Name Transcription Factor Ap-4
Activating Enhancer-Binding Protein 4
Class C Basic Helix-Loop-Helix Protein 41
Bhlhc41
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Ap-4 antibody
Anti-Activating Enhancer-Binding Protein 4 antibody
Anti-Class C Basic Helix-Loop-Helix Protein 41 antibody
Anti-Bhlhc41 antibody
Anti-TFAP4 antibody
Anti-BHLHC41 antibody

Information sourced from Uniprot.org

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