![Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98011/381531/STJ119138_1__83947.1713149676.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98011/381532/STJ119138_2__47859.1713149677.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and TFAP4 knockout (KO) HeLa cells, using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98011/381533/STJ119138_3__96167.1713149677.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TFAP4 knockout (KO) HeLa cells, using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60S.")
![Western blot analysis of various lysates using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98011/381534/STJ119138_4__15984.1713149678.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98011/381531/STJ119138_1__83947.1713149676.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-TFAP4 antibody (200-338) (STJ119138)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TFAP4 (200-338) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TFAP4 |
| Gene ID | 7023 |
| Uniprot ID | TFAP4_HUMAN |
| Immunogen Region | 200-338 |
| Immunogen Sequence | QQEQVRLLHQEKLEREQQQL RTQLLPPPAPTHHPTVIVPA PPPPPSHHINVVTMGPSSVI NSVSTSRQNLDTIVQAIQHI EGTQEKQELEEEQRRAVIVK PVRSCPEAPTSDTASDSEAS DSDAMDQSREEPSGDGELP |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-338 of human TFAP4 (NP_003214.1). |
Additional Info
| Function | Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'. |
| Protein Name | Transcription Factor Ap-4Activating Enhancer-Binding Protein 4Class C Basic Helix-Loop-Helix Protein 41Bhlhc41 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Ap-4 antibodyAnti-Activating Enhancer-Binding Protein 4 antibodyAnti-Class C Basic Helix-Loop-Helix Protein 41 antibodyAnti-Bhlhc41 antibodyAnti-TFAP4 antibodyAnti-BHLHC41 antibody |
Information sourced from Uniprot.org