Anti-TCF3 antibody (571-620 aa) (STJ95944)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TCF3 at the amino acid range 571-620
General Information
| Short Description | Rabbit polyclonal anti-Transcription factor E2-alpha (571-620 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TCF3 |
| Gene ID | 6929 |
| Uniprot ID | TFE2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TCF3 at the amino acid range 571-620 |
| Immunogen Region | 571-620 aa |
| Specificity | TCF-3 Polyclonal Antibody detects endogenous levels of TCF-3 protein. |
Additional Info
| Function | Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. Isoform E47: Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity. |
| Protein Name | Transcription Factor E2-AlphaClass B Basic Helix-Loop-Helix Protein 21Bhlhb21Immunoglobulin Enhancer-Binding Factor E12/E47Immunoglobulin Transcription Factor 1Kappa-E2-Binding FactorTranscription Factor 3Tcf-3Transcription Factor Itf-1 |
| Database Links | Reactome: R-HSA-525793Reactome: R-HSA-8939236Reactome: R-HSA-9839394 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2-Alpha antibodyAnti-Class B Basic Helix-Loop-Helix Protein 21 antibodyAnti-Bhlhb21 antibodyAnti-Immunoglobulin Enhancer-Binding Factor E12/E47 antibodyAnti-Immunoglobulin Transcription Factor 1 antibodyAnti-Kappa-E2-Binding Factor antibodyAnti-Transcription Factor 3 antibodyAnti-Tcf-3 antibodyAnti-Transcription Factor Itf-1 antibodyAnti-TCF3 antibodyAnti-BHLHB21 antibodyAnti-E2A antibodyAnti-ITF1 antibody |
Information sourced from Uniprot.org