| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Transcription factor E2-alpha (571-620 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TCF3 |
| Gene ID: | 6929 |
| Uniprot ID: | TFE2_HUMAN |
| Immunogen Region: | 571-620 aa |
| Specificity: | TCF-3 Polyclonal Antibody detects endogenous levels of TCF-3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human TCF3 at the amino acid range 571-620 |
| Function | Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. Isoform E47: Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity. |
| Protein Name | Transcription Factor E2-Alpha Class B Basic Helix-Loop-Helix Protein 21 Bhlhb21 Immunoglobulin Enhancer-Binding Factor E12/E47 Immunoglobulin Transcription Factor 1 Kappa-E2-Binding Factor Transcription Factor 3 Tcf-3 Transcription Factor Itf-1 |
| Database Links | Reactome: R-HSA-525793 Reactome: R-HSA-8939236 Reactome: R-HSA-9839394 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2-Alpha antibody Anti-Class B Basic Helix-Loop-Helix Protein 21 antibody Anti-Bhlhb21 antibody Anti-Immunoglobulin Enhancer-Binding Factor E12/E47 antibody Anti-Immunoglobulin Transcription Factor 1 antibody Anti-Kappa-E2-Binding Factor antibody Anti-Transcription Factor 3 antibody Anti-Tcf-3 antibody Anti-Transcription Factor Itf-1 antibody Anti-TCF3 antibody Anti-BHLHB21 antibody Anti-E2A antibody Anti-ITF1 antibody |
Information sourced from Uniprot.org
