Western blot analysis of various lysates using TBX5 Rabbit polyclonal antibody (STJ118041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TBX5 antibody (1-70) (STJ118041)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ118041
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General Information

Short DescriptionRabbit polyclonal TBX5 (1-70) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTBX5
Gene ID6910
Uniprot IDTBX5_HUMAN
Immunogen Region1-70
Immunogen SequenceMADADEGFGLAHTPLEPDAK DLPCDSKPESALGAPSKSPS SPQAAFTQQGMEGIKVFLHE RELWLKFHEV
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TBX5 (NP_852259.1).

Additional Info

Post Translational Modifications Acetylation at Lys-339 by KAT2A and KAT2B promotes nuclear retention.
Function DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter.
Protein Name T-Box Transcription Factor Tbx5
T-Box Protein 5
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-5578768
Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Cytoplasm And The Nucleus
Acetylation At Lys-339 Promotes Nuclear Retention
Alternative Antibody Names Anti-T-Box Transcription Factor Tbx5 antibody
Anti-T-Box Protein 5 antibody
Anti-TBX5 antibody

Information sourced from Uniprot.org

Citations

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