Anti-TAP2 antibody (450-686) (STJ25775)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TAP2 (450-686) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TAP2 |
| Gene ID | 6891 |
| Uniprot ID | TAP2_HUMAN |
| Immunogen Region | 450-686 |
| Immunogen Sequence | QPNLPSPGTLAPTTLQGVVK FQDVSFAYPNRPDRPVLKGL TFTLRPGEVTALVGPNGSGK STVAALLQNLYQPTGGQVLL DEKPISQYEHCYLHSQVVSV GQEPVLFSGSVRNNIAYGLQ SCEDDKVMAAAQAAHADDFI QEMEHGIYTDVGEKGSQLAA GQKQRLAIARALVRDPRVLI LDEATSALDVQCEQALQDWN SRGDRTVLVIAHRLQTVQRA HQILVLQEGKLQKLAQL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 450-686 of human TAP2 (NP_001276972.1). |
Additional Info
| Function | ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation. |
| Protein Name | Antigen Peptide Transporter 2Apt2Atp-Binding Cassette Sub-Family B Member 3Peptide Supply Factor 2Peptide Transporter Psf2Psf-2Peptide Transporter Tap2Peptide Transporter Involved In Antigen Processing 2Really Interesting New Gene 11 ProteinRing11 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinThe Transmembrane Segments Seem To Form A Pore In The Membrane |
| Alternative Antibody Names | Anti-Antigen Peptide Transporter 2 antibodyAnti-Apt2 antibodyAnti-Atp-Binding Cassette Sub-Family B Member 3 antibodyAnti-Peptide Supply Factor 2 antibodyAnti-Peptide Transporter Psf2 antibodyAnti-Psf-2 antibodyAnti-Peptide Transporter Tap2 antibodyAnti-Peptide Transporter Involved In Antigen Processing 2 antibodyAnti-Really Interesting New Gene 11 Protein antibodyAnti-Ring11 antibodyAnti-TAP2 antibodyAnti-ABCB3 antibodyAnti-PSF2 antibodyAnti-RING11 antibodyAnti-Y1 antibody |
Information sourced from Uniprot.org