| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TAP2 (450-686) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TAP2 |
| Gene ID: |
6891 |
| Uniprot ID: |
TAP2_HUMAN |
| Immunogen Region: |
450-686 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 450-686 of human TAP2 (NP_001276972.1). |
| Immunogen Sequence: |
QPNLPSPGTLAPTTLQGVVK FQDVSFAYPNRPDRPVLKGL TFTLRPGEVTALVGPNGSGK STVAALLQNLYQPTGGQVLL DEKPISQYEHCYLHSQVVSV GQEPVLFSGSVRNNIAYGLQ SCEDDKVMAAAQAAHADDFI QEMEHGIYTDVGEKGSQLAA GQKQRLAIARALVRDPRVLI LDEATSALDVQCEQALQDWN SRGDRTVLVIAHRLQTVQRA HQILVLQEGKLQKLAQL |
| Function | ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation. |
| Protein Name | Antigen Peptide Transporter 2Apt2Atp-Binding Cassette Sub-Family B Member 3Peptide Supply Factor 2Peptide Transporter Psf2Psf-2Peptide Transporter Tap2Peptide Transporter Involved In Antigen Processing 2Really Interesting New Gene 11 ProteinRing11 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinThe Transmembrane Segments Seem To Form A Pore In The Membrane |
| Alternative Antibody Names | Anti-Antigen Peptide Transporter 2 antibodyAnti-Apt2 antibodyAnti-Atp-Binding Cassette Sub-Family B Member 3 antibodyAnti-Peptide Supply Factor 2 antibodyAnti-Peptide Transporter Psf2 antibodyAnti-Psf-2 antibodyAnti-Peptide Transporter Tap2 antibodyAnti-Peptide Transporter Involved In Antigen Processing 2 antibodyAnti-Really Interesting New Gene 11 Protein antibodyAnti-Ring11 antibodyAnti-TAP2 antibodyAnti-ABCB3 antibodyAnti-PSF2 antibodyAnti-RING11 antibodyAnti-Y1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
