• Immunofluorescence analysis of U-2 OS cells using TACC3 Rabbit monoclonal antibody (STJ11103055) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TACC3 Rabbit monoclonal antibody (STJ11103055) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TACC3 in paraffin-embedded human tonsil tissue using TACC3 Rabbit monoclonal antibody (STJ11103055) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TACC3 in paraffin-embedded human colon tissue using TACC3 Rabbit monoclonal antibody (STJ11103055) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from 293T cells, using TACC3 Rabbit monoclonal antibody (STJ11103055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TACC3 antibody (1-100) [S5MR] (STJ11103055)

SKU:
STJ11103055

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TACC3 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TACC3
Gene ID: 10460
Uniprot ID: TACC3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TACC3 (NP_006333.1).
Immunogen Sequence: MSLQVLNDKNVSNEKNTENC DFLFSPPEVTGRSSVLRVSQ KENVPPKNLAKAMKVTFQTP LRDPQTHRILSPSMASKLEA PFTQDDTLGLENSHPVWTQK
Function Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.
Protein Name Transforming Acidic Coiled-Coil-Containing Protein 3
Eric-1
Database Links Reactome: R-HSA-9013507
Reactome: R-HSA-9604323
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Spindle Pole
In Complex With Ckap5 Localized To Microtubule Plus-Ends In Mitosis And Interphase
In Complex With Ckap5 And Clathrin Localized To Inter-Microtubule Bridges In Mitotic Spindles
Alternative Antibody Names Anti-Transforming Acidic Coiled-Coil-Containing Protein 3 antibody
Anti-Eric-1 antibody
Anti-TACC3 antibody
Anti-ERIC1 antibody

Information sourced from Uniprot.org

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