Anti-SYMPK antibody (310-530) (STJ111388)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111388
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General Information

Short DescriptionRabbit polyclonal SYMPK (310-530) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSYMPK
Gene ID8189
Uniprot IDSYMPK_HUMAN
Immunogen Region310-530
Immunogen SequenceKHPASLEFQAQITTLLVDLG TPQAEIARNMPSSKDTRKRP RDDSDSTLKKMKLEPNLGED DEDKDLEPGPSGTSKASAQI SGQSDTDITAEFLQPLLTPD NVANLVLISMVYLPEAMPAS FQAIYTPVESAGTEAQIKHL ARLMATQMTAAGLGPGVEQT KQCKEEPKEEKVVKTESVLI KRRLSAQGQAISVVGSLSSM SPLEEEAPQAKRRPEPIIPV T
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 310-530 of human SYMPK (NP_004810.2).

Additional Info

Tissue Specificity In testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. The protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node.
Function Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.
Protein Name Symplekin
Database Links Reactome: R-HSA-159231
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Tight Junction
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Nucleus
Nucleoplasm
Cytoplasmic Face Of Adhesion Plaques (Major) And Nucleoplasm (Minor) (In Cells With Tj)
Nucleoplasm (In Cells Without Tj)
Nuclear Bodies Of Heat-Stressed Cells
Colocalizes With Hsf1 In Nuclear Stress Bodies Upon Heat Shock
Alternative Antibody Names Anti-Symplekin antibody
Anti-SYMPK antibody
Anti-SPK antibody

Information sourced from Uniprot.org

Citations

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